C (3-6 months) C（3〜6か月） Developed by Hiroki Kurihara, Graduate School of Medicine, University of Tokyo in 2001. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ res., 89, 983-990 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. 開発者/機関 : 栗原裕基先生 (東京大学)開発年 : 2001年その他 (入手経路等) : 自家樹立129/Sv×C57BL/6系統。129/Sv-derived ES細胞　SM-1を用いて作出。その後，C57BL/6との交配にて維持。 <a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/04109_GB.pdf">Genetic Background</a> 戻し交配系統名 (ブリーダー名) : C57BL/6J (日本クレア)特記 : ホモ接合体にて維持。 true RBRC04109 戻し交配 系統名 (ブリーダー名) : C57BL/6J (日本クレア) 特記 : ホモ接合体にて維持。 The alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT. The elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability. Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Calca genomic DNA alphaCGRP- alphaCGRP- Hiroki KURIHARA The alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT. The alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT. The elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> B6.129S6-Calca<tm1Hku> B6.129S6-Calca<tm1Hku> SM1[129S6/SvEvTac] 開発者/機関 : 栗原裕基先生 (東京大学) 開発年 : 2001年 その他 (入手経路等) : 自家樹立 129/Sv×C57BL/6系統。129/Sv-derived ES細胞　SM-1を用いて作出。 その後，C57BL/6との交配にて維持。 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Circ res., 89, 983-990 (2001).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾書を得ること。 <a href='https://brc.riken.jp/mus/pcr04109'>Genotyping protocol -PCR-</a> 栗原　裕基